qPCR 2011 Symposium in Freising

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Poster number    Poster session

P001 – P046        New qPCR Applications: Molecular Diagnostics & Expression Profiling
P047 – P055        New qPCR Applications: Method Optimisation & Standardisation
P056 – P061        qPCR Biostatistics & Bioinformatics
P062 – P069        High Throughput qPCR & digital PCR Next Genartion Sequencing  
P070 – P075        Single-cell qPCR qPCR from limited material  
P076 – P084        HRM & Methylation Studies  
P085 – P090        RNAi:  microRNA – siRNA Applications  
P091                    late submission  


P001 – P046    New qPCR Applications: Molecular Diagnostics & Expression Profiling

P001 - PCR applications for diagnosis in Animal Health
Jovita Fernandez-Pinero1, Montserrat Agüero2 1Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, Spain; 2Laboratorio Central de Veterinaria (LCV, MARM), Algete, Spain
P002 - A Comparative qPCR Analysis of Apoptotic Genes in Milk Somatic Cells of Cows with history of Inconsistent Lactations
Nishant Varshney1, Surender Singh1, Manu Jamwal1, Sudarshan Kumar1, Dhruba Malakar1, Jai Kumar Kaushik1, Sunita Grover1, B S Prakash1, Bishnu P Mishra2, Tapas Mukhopadhyay3, Ajay K Dang1, Ashok K Mohanty1
1National Dairy Research Institute, Karnal, India; 2National Bureau of Animal Genetic Resources, Karnal, India; 3National Centre for Human Genome Studies and Research, Punjab University, Chandigarh, India
P003 - Constitutive expression of HSPA-1A gene in two different sources of buffalo (Bubalus bubalis) embryos
Nath Amar, Saikumar G., Gade Nitin Eknath, Taru Sharma G. Indian Vetrinary Research Institute, India
P004 - Applicability of qPCR to ensure the quality of milk and dairy products
C LEMAITRE, E LABBE, T RIBEIRO, A CHANGO, A ABDEL NOUR LaSalle Beauvais, France
P005 - Expression study of developmental genes in in vitro produced buffalo (Bubalus bubalis) embryos generated by hand-made cloning and in vitro fertilization
SUDEEPTA KUMAR PANDA, KARN PRATAP SINGH, AMAN GEORGE, AMBIKA PRASSNA SAHA, RUCHI SHRAMA, RADHEY SHAM MANIK, MANMOHAN SINGH CHAUHAN, PRABHAT PALTA, SURESH KUMAR SINGLA
NATIONAL DAIRY RESAERCH INSTITUTE, India
P006 - Validation of RT-qPCR measurements in primary bovine mammary gland epithelial cells (pbMEC)
Diana Sorg, Anne Potzel, Heinrich H. D. Meyer, Enrique Viturro, Heike Kliem Technische Universität München, Germany
P007 - Heterogeneity within the pluripotent stem cell state is associated with transient changes in gene expression
Janice K Au-Young1, Jason Gioia1, Srividya Dadi1, David Keys1, Paul J Gokhale2, Peter Andrews2 1Life Technologies, United States of America; 2Centre for Stem Cell Biology, University of Sheffield, UK
P008 - Impact of long-term expansion on mesenchymal stromal cell differentiation potential
Andrea Hecker, Irena Brinkmann, Karen Bieback Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim
P009 - Standardisation of triplex real time RT-PCR for detection and quantification of hepatitis E virus
Petra Vasickova, Petr Kralik, Iva Slana, Ivo Pavlik Veterinary Research Institute, Czech Republic
P010 - Molecular basis for the impaired adipogenic differentiation potential of cord-blood-derived mesenchymal stromal cells
Irena Brinkmann, Andrea Hecker, Marianna Karagianni, Karen Bieback
Institute of Transfusion Medicine and Immunology, Germany
P011 - qPCR assay for detection of human faecal contamination in food samples
Lotte Bjerrum1, Anna Charlotte Schultz2, Dorthe Lau Baggesen2, Anna Krestine Nørgaard1, Aaron Marc Saunders3 1Danish Technological Institute, Denmark; 2National Food Institute, Technical University of Denmark; 3Aalborg University, Denmark
P012 - qPCR method to calculate the copy number of IS1111 elements in Coxiella burnetii
Matthias Hanczaruk, Anke Stark, Dimitrios Frangoulidis Bundeswehr Institute of Microbiology, Germany
P013 - Quality assurance in GMO analysis – A practical approach for estimation of measurement uncertainty and visualisation of quantitative real-time PCR data
Lars Gerdes, Wilhelm Dicke, Ulrich Busch, Sven Pecoraro Bavarian Health and Food Safety Authority (LGL), Germany
P014 - Quantitative Real Time PCR in Friedreich’s Ataxia: implications for diagnosis and clinical trial design.
Giorgia Puorro1, Antonella Antenora1, Angela Marsili1, Alessandra Denaro1, Raffaele Piro1, Pierpaolo Sorrentino1, Chiara Pane1, Alessandra Tessa2, Sergio Cocozza3, Filippo Santorelli2, Giuseppe De Michele1, Alessandro Filla1, Francesco Saccà1 1Dipartimento di Scienze Neurologiche, University Federico II, Naples, Italy; 2Dipartimento Clinico di Neuroscienze e dell'Età Evolutiva, IRCCS Stella Maris, Pisa, Italy; 3Dipartimento di Biologia Cellulare e Molecolare, University Federico II, Naples, Italy
P015 - Cutaneous Mycobacterium marinum infection in humans detected by quantitative real time PCR
Michal Slany1, Krystyna Kantorova1, Petr Jezek2, Monika Bodnarova3, Ivo Pavlik1 1Veterinary research institute, Czech Republic; 2County Hospital Pribram, Czech Republic; 3Charles University Praque, First Faculty of Medicine and General University Hospital, Czech Republic
P016 - Quantization of Rauscher-like MuLV in plasma from BALB/cJ mice
Yehuda Stram1, Marisol Rubinstein1, Evgenia Lubashevsky1, Sofa Savranski1, Gavriel Leitner1, Zeev Treinin2 1Kimron Veterinary Institute, Israel; 2Israel Dairy Board
P017 - Development of a real-time quantitative multiplex TaqMan-based RT-PCR assay for detection and quantification of measles, mumps and rubella viruses
Yulia Zabiyaka, Eugeny Faizuloev, Sergey Lobodanov, Tatiana Konstantinovna Borisova
Mechnikov Research Institute of Vaccines and Sera, RAMS, Russian Federation
P018 - Comparative study of direct sequencing and TheraScreen assay for EGFR mutational analysis
LUZ MARTINEZ AVILES, JAVIER GIMENO, RAQUEL LONGARON, ERICA TORRES, GEMMA NAVARRO, LARA PIJUAN, SERGI SERRANO, BEATRIZ BELLOSILLO HOSPITAL DEL MAR, Spain
P019 - Real-time PCR with SYBR Green and melting curve analysis as a diagnostic screening tool for protozoan oocysts
Laura F. Lalonde, Alvin A. Gajadhar Canadian Food Inspection Agency, Canada
P020 - RNA isolation from high-sugar content freeze-dried tomato (Solanum lycopersicum L.) fruits and qRT-PCR analysis
Libert Brice TONFACK1,2, Emmanuel YOUMBI1, Benoit van-der-Rest2, Alain LATCHE2, Jean-Claude PECH2 1University of Yaoundé I, Cameroon; 2UMR990, Genomic et Biotechnologie des Fruits INRA/INP-ENSAT, Toulouse, France
P021 - Seasonal quantification of Grapevine fanleaf virus by one-step RT real-time PCR
Urska Cepin, Ion Gutiérrez-Aguirre, Maruša Pompe-Novak, Kristina Gruden, Maja Ravnikar National Institute of Biology, Slovenia
P022 - Submicroscopic duplications identified during prenatal aneuploidy testing
Alison Hills1, Kathy Mann1, Caroline Mackie Ogilvie2 1GSTS Pathology, Guys and St Thomas NHS Foundation Trust, United Kingdom; 2Cytogenetics Department, Guys and St Thomas NHS Foundation Trust, United Kingdom
P023 - The Design and Construction of Competitor Fragment by SOE-PCR for Quantification of The Ruminal Bacterium: Butyrivibrio fibrisolvens
Amir Taheri Ghahfarokhi, Mojtaba Tahmoorespur, Mohammad Reza Nassiry, Mohammad Hadi Sekhavati Ferdowsi University of Mashhad, Mashhad, Iran
P024 - A Modular approach in method validation of the real-time PCR module for the detection of five VTEC Escherichia coli serogroups
Dafni-Maria Kagkli1, Thomas P. Weber1, Marc Van den Bulcke1, Silvia Folloni1, Stefano Morabito2, Rosangela Tozzoli2, Monica Ermolli1, Laura Gribaldo1, Guy Van den Eede1
11European Commission Joint Research Centre, Institute for Health and Consumer Protection, Molecular Biology and Genomics Unit, via E. Fermi 2749, I-21027, Ispra (VA), Italy; 2Istituto Superiore di Sanità, Viale Regina Elena 299, I-00161, Rome, Italy
P025 - Comparative study of direct sequencing and TheraScreen assay for KRAS mutational analysis
LUZ MARTINEZ AVILES, MAR IGLESIAS, ESTER MORAGON, ERICA TORRES, GEMMA NAVARRO, MAR GARCIA GARCIA, SERGI SERRANO, BEATRIZ BELLOSILLO HOSPITAL DEL MAR, Spain
P026 - Developing an optimal qRT-PCR strategy for fungal transcriptomics on carotenoid metabolism
Yamuna Sahadevan1, Wilhelm Boland1, Kerstin Hoffmann2, Kerstin Voigt2
1Dept.of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology,Jena, Germany; 2Jena Microbial Resource Collection, Dept.of Microbiology and Molecular Biology,University of Jena and Hans-Knöll-Institute, Jena,Germany
P027 - Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species
Alexander Nagy1, Veronika Vostinakova1, Zuzana Pirchanova1, Lenka Cernikova1, Zuzana Dirbakova2, Miroslav Mojzis2, Helena Jirincova3, Martina Havlickova3, Adam Dan4, Krisztina Ursu4, Stefan Vilcek5, Vlastimil Krivda1, Jitka Hornickova1
1State Veterinary Institute Prague, National Reference Laboratory for Avian Influenza and Newcastle Disease, Czech Republic; 2State Veterinary Institute Zvolen, National Reference Laboratory for Avian Influenza and Newcastle Disease, Zvolen, Slovak Republic; 3National Institute of Public Health, National Reference Laboratory for Human Influenza, Prague, Czech Republic; 4Central Agricultural Office, Veterinary Diagnostic Directorate, Molecular Biology Laboratory, Budapest, Hungary; 5University of Veterinary Medicine in Kosice, Department of Parasitology and Infectious Diseases, Kosice, Slovak Republic
P028 - Dietary fibers are not born equal: qPCR to define prebiotic properties
F DEPEINT, G ABDEL NOUR, CN NIAMBA, P POUILLART, A ABDEL NOUR LaSalle Beauvais, France
P029 - Disrupted balance between phase I and phase II estrogen-metabolising enzymes may contribute to the growth of endometriotic tissue in ovarian endometriosis
Neli Hevir1, Martina Ribič-Pucelj2, Tea Lanišnik Rižner1 1Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia; 2Department of Obstetrics and Gynaecology, University Medical Centre, Ljubljana, Slovenia
P030 - Evaluation of Various Concentration Methods for Detecting Enterovirus in Water
Bing-Mu Hsu National Chung Cheng University, Taiwan, Republic of China
P031 - Event-specific detection of genetically modified rice (Kefeng 6) by means of quantitative real-time PCR
Patrick Guertler, Ingrid Huber, Sven Pecoraro, Ulrich Busch Bavarian Health and Food Safety Authority, Germany
P032 - Examining resistance gene expression using reverse transcriptase qPCR
Tine Yding Wolff1, Jan Lorenzen1, Trine Rolighed Thomsen1,2, Anne Karin Ildor Rasmussen3, Henrik Stender3, Helle Stendahl Andersen1, Masumeh Chavoshi1
1The Danish Technological Institute, Denmark; 2Aalborg University, Denmark; 3AdvanDx, Denmark
P033 - Experience with Plant RNA Isolation Aid for the total RNA isolation from plants
Irena Mavric Plesko1, Mojca Virscek Marn1, Natasa Toplak2, Minka Kovac2 1Kmetijski institut Slovenije, Slovenia; 2Omega d.o.o., Slovenia
P034 - Expression of Laccase genes in Schizophyllum commune
Soumya Madhavan, Katrin Krause, Erika Kothe Friedrich Schiller University, Institute of Microbiology, Microbial Phytopathology, D-07743 Jena, Germany
P035 - Expression of local luteotropic factors during induced luteolysis in the bovine corpus luteum
Dieter Schams1, Heinrich Meyer1, Bajram Berisha1,2 1Physiology Weihenstephan, Technische Universität München, Germany; 2Faculty of Agriculture and Veterinary, University of Prishtina, Kosovo
P036 - Expresssion of Adrenomedullin signalling factors in bovine corpus luteum during oestrous cycle, pregnancy and induced luteolysis
Bajram Berisha1,2, Stefanie Schilffarth1, Mihir Sarkar1, Dieter Schams1 1Physiology Weihenstephan, Technische Universität München, Germany; 2Faculty of Agriculture and Veterinary, University of Prishtina, Kosovo
P037 - Gene Expression of Selected WRKY Transcription Factors in Arabidopsis thaliana Cell Cultures during a Parabolic Flight
Anne Hennig, Maren Neef, Niklas Hausmann, Rüdiger Hampp University of Tuebingen, Physiological Ecology of Plants, Germany
P038 - Gene expression studies on soil fertility and nutritional status in grapevine
Nadia Bertazzon1, Piergiorgio Stevanato2, Alessandro Zonin2, Giuseppe Concheri2, Massimo Saccomani2, Elisa Angelini1 1C.R.A. Centro di Ricerca per la Viticoltura, 31015, CONEGLIANO (TV), Italy; 2Università degli Studi di Padova, Dipartimento di Biotecnologie Agrarie, 35020, LEGNARO (PD), Italy
P039 - H275Y screening and quantification using isolated unlabeled probe
Hong Kai Lee1, Chun Kiat Lee1, Julian Wei-Tze Tang1, Evelyn Siew-Chuan Koay1,2
1Molecular Diagnosis Centre, Department of Laboratory Medicine, National University Hospital, Singapore; 2Department of Pathology, National University of Singapore, Singapore
P040 - Improvement of phytoplasma diagnostics in grapevine and fruit trees: from grinding to real time PCR analysis
Matevž Rupar, Nina Prezelj, Nataša Mehle, Petra Nikolić, Maja Ravnikar, Marina Dermastia National Institute of Biology, Slovenia
P041 - Influence of different one-step reverse transcription real-time PCR reagents for no-doubt detection of norovirus genogroup II
Marko Kolenc1, Nataša Toplak2, Minka Kovač2, Andrej Steyer1, Mateja Poljšak Prijatelj1 1University of Ljubljana, Faculty of Medicine, Institute of Microbiology and Immunology, Slovenia; 2Omega d.o.o., Slovenia
P042 - Interest of Real-Time PCR for the Diagnosis of Invasive Aspergillosis
Inès Hadrich1, Fattouma Makni1, Sourour Neji1, Fatma Cheikhrouhou1, Hayet Sellami1, Stéphane Ranque2, Ali Ayadi1 1Habib Bourguiba Hospital: Sfax - Tunisia, Tunisia; 2Laboratoire de Parasitologie-Mycologie, Université de la Méditerranée, AP-HM Timone, Marseille France
P043 - Is the JAK2 Exon 14 Deletion a Physiological Splice Variant?
Paolo Catarsi, Vittorio Rosti, Laura Villani, Valentina Poletto, Elisa Bonetti, Bergamaschi Gaetano, Barosi Giovanni Unit of Clinical Epidemiology and Center for the Study of Myelofibrosis, IRCCS Policlinico San Matteo Foundation, Italy
P044 - Molecular Detection of Tranzschelia discolor using qPCR
Paolo Catarsi1,3, Cristina Borghi1, Marina Laura1, Arianna Cassetti1, Giacomo Morreale2, Andrea Allavena1 1CRA-FSO, Research Unit for Floriculture and Ornamental Species, Corso Inglesi 508, 18038 Sanremo, Italy; 2CRA – VIT, Centre for Research in. Viticulture, Viale XXVIII Aprile 26, 31015, Conegliano, Italy; 3Current address: Lab of Clinical Epidemiology, IRCCS Policlinico San Matteo Foundation, Piazzale Golgi, 27100 Pavia, Italy
P045 - Scaling Semiconductor Sequencing from Microbial to Human Genomes on a Single Platform
Alain Rico1, Jonathan M. Rothberg2 1Life Technologies, France; 2Ion Torrent, USA
P046 - Multiplex Real-Time PCR for Detection of Shiga toxin and Intimin genes of pathogenic Escherichia coli
Melanie Pavlovic, Regina Konrad, Sabine Wolf, Marion Lindermayer, Jasmin Fräßdorf, Ulrich Busch, Ingrid Huber
Bavarian Health and Food Safety Authority, Germany


P047 – P055    New qPCR Applications: Method Optimisation & Standardisation

P047 Hot Start dNTPs – Novel Chemistries for Use in Advanced PCR Applications
Natasha Paul TriLink BioTechnologies, Inc., United States of America
P048 - Lyophilized Mix of Enzyme, Probes, and Primers: A simple and highly sensitive solution for qPCR applications.
Nanette Umblas, Chieh-Yuan Li, Lin Lin Huang, Kathy Lee, David Keys, Kate Hames, Madhu Augustine, Stephen Hendricks Life Technologies, United States of America
P049 - Extraction protocol to increase the yield of circulating fetal DNA in maternal plasma
Lai-on Chu, C.Y. Rachel Yeung, K.F. Joseph Chow DiagCor Bioscience Inc. Ltd., Hong Kong S.A.R. - China
P050 - Quantitative detection of pathogens and of mRNA/gene mutants: Internal and external reference RNAs and DNAs for the development and high level quality & performance controls of real-time RT-PCR and PCR assays
Guido Krupp, Andreas Hanne, Peter Scheinert AmpTec GmbH, Germany
P051 - Validation of appropriate reference genes for the normalization of real-time quantitative RT-PCR data obtained from platelets of post-myocardial infarction patients
Amir Houshang Shemirani1,2, Katalin Szilvia Zsóri1,2, László Muszbek1 1Debrecen University, Hungary; 2Sátoraljaújhely hospital, Hungary
P052 - Exogenous RNA control for indication of reaction inhibition in the RT-qPCR workflow
James Covino2, Zaklina Strezoska2, Devin Leake2, Annaleen Vermeulen2, Melissa Kelley2, Jaakko Kurkela1 1Thermo Fisher, Finland; 2Thermo Fisher Scientific, Lafayette, Colorado
P053 - Internal controls for quantification of alternatively spliced transcripts by real-time PCR
Julia Meiser, Julia Frühwald, Stephan E. Philipp Institute for experimental and clinical pharmacology and toxicology, University Saarland, 66421 Homburg, Germany
P054 - Contamination control with the heat-labile cod UNG ensures accurate and reliable results in your post-PCR analysis
Elisabeth Lill Andreassen, Olav Lanes, Morten Elde ArcticZymes AS, Norway
P055 - NOVEL APPROACHES IN MANUFACTURING HIGH QUALITY REAGENTS FOR IN-HOUSE PCR DIAGNOSTICS
Muriel Craynest EUROGENTEC, Belgium


P056 – P061    qPCR Biostatistics & Bioinformatics

P056 - Real-time PCR quantifications in presence of inhibitors using SOD and Cy0 methods.
Davide Sisti, Michele Guescini, Renato Panebianco, Pasquale Tibollo, Michela Mantuano, Marco Rocchi, Vilberto Stocchi University of Urbino, Italy
P057 - Kinetic models of qPCR as applied to the problem of low copy numbers
Alexander M Chagovetz, James P Keener University of Utah, United States of America
P058 - Modeling Immuno-qPCR to Estimate Protein Quantity
Harrison Leong, elana swartzman, jennifer holmdahl Life Technologies, United States of America
P059 - Development of a GMO screening database with combinatory algorithms and its application in official food and feed control
Lars Gerdes, Sven Pecoraro Bavarian Health and Food Safety Authority (LGL), Germany
P060 - Advanced copy number variant analysis with qbasePLUS
Barbara D'haene, Jo Vandesompele, Jan Hellemans Biogazelle, Zwijnaarde, Belgium
P061 - Full-Process-Kinetics PCR analysis: a holistic model to PCR data interpretation.
Antoon Lievens1, Stefan Van Aelst2, Marc Van den Bulcke1, Els Goetghebeur2 1Institute of Public Health, Belgium; 2Ghent University, Belgium


P062 – P069    High Throughput qPCR & digital PCR Next Genartion Sequencing

P062 - Identification of Tumor Cell Lines by Molecular Signature using Digital PCR and TaqMan® Copy Number Assays
Julie Kuykendall Life Technologies, United States of America
P063 - High throughput microfluidic dynamic array profiling demonstrates differential gene expression of immune related genes during early pregnancy in cattle
Anna E Groebner1, Katy Schulke1, Horst D Reichenbach2, Heinrich HD Meyer1, Susanne E Ulbrich1
1Physiology Weihenstephan, Technische Universitaet Muenchen, Germany; 2Bavarian State Research Center for Agriculture, Institute of Animal Breeding, Grub, Germany
P064 - RealTime ready qPCR Assays - Workflow and an interim census of 10k+ functionally tested qPCR assays
Christine Bruns, Manuel Dietrich, Ursula Grepl, Rita Haerteis, Ingrid Hoffmann, Regina Huber, Bastian Kramer, Irene Labaere, Ralf Mauritz, Elke Stadlbauer, Heiko Walch Roche Applied Science, Germany
P065 - Parallel Preparation of Targeted Resequencing Libraries from 480 Genomic Regions Using Multiplex PCR on the Access Array™ System
Fiona Kaper1, Jun Wang, Megan J. Anderson, Peilin Chen, Min Min Lin, Martin Pieprzyk, Robert C. Jones, Andrew P. May Fluidigm Corporation, 7000 Shoreline Court, Suite 100, South San Francisco, CA 94080
P066 - Integration of a novel real-time PCR detection system onto Luminex beads in a PCR reaction.
Ross Thomas Barnard1, Richard Lai1, Simon Corrie2, Che-Cheng Yeh1, Graeme Barnett3, John Feros3 1School of Chemistry and Molecular Biosciences,The University of Queensland, Australia; 2Australian Institute for Bioengineering and Nanotechnology, The University of Queensland; 3BioChip Innovations Pty. Ltd., Suite 4, 1407 Logan Rd, Mt Gravatt, Qld 4122, Australia
P067 - Performance and Accuracy Breakthroughs with the 5500 Series SOLiD™ Sequencers
Oliver Stephan1, Timothy Burcham1, Patrick Kinney1, Steve Boege1, Jon Hoshizaki1, Matt Chan1, David Cox1, Jason Briggs1, Lee Jones1, Janet Ziegle1, Larry Joe1, Sylvia Chang1, Kathleen Perry1, Takuya Matsui2, Shuhei Yamamoto2, Ryoji Inaba2 1Lifetechnologies, Inc; 2Hitachi High Technologies, Hitachinaka-shi, Ibaraki-ken, Japan
P068 - qPCR in immune system monitoring : a high throughput method
Maxime Lilian Dooms, Flore Depeint, Afif M Abdel Nour LaSalle Beauvais, France
P069 - Transcriptome analyses in rice: validation of mRNAseq using qRT-PCR
Tina Kyndt1, Geert Trooskens1, Simon Denil1, Tim Demeyer1, Annelies Haegeman1, Wim Van Criekinge1,2, Godelieve Gheysen1 1Department of Molecular Biotechnology, UGent, Belgium; 2NXTGNT, UGent, Belgium


P070 – P075                Single-cell qPCR qPCR from limited material

P070 - At the single cell level cellular noise challenges normalization
Richard Fekete, Ron Abruzesse, Elena Grigorenko Life Technologies, United States of America
P071 - Two different astrocytes subpopulation in cortex of mice
Vendula Rusnakova1, Pavel Honsa2, Miroslava Anděrová2, Mikael Kubista1 1Institure of Biotechnology, Czech Republic; 2Isntitute of Experimental Medicine
P072 - Gene expression analysis of formalin-fixed paraffin embedded laser microdissected endometrial tissue sections
Anna E Groebner1, Nevena Kirova1, Harald Welter2, Artur Mayerhofer2, Heinrich HD Meyer1, Susanne Ulbrich1
1Physiologie Weihenstephan, Technische Universitaet Muenchen, Germany; 2Institute for cell biology, Ludwig Maximilian Universitaet Muenchen, Germany
P073 - Assessing heterogeneity at a single cell level in a clonal population of transformed epidermal keratinocytes
RM Teisanu1, H Richter2, J Moore3, A Paillusson2, O Haglebüchle2, J Weber2, K Harshman2, Y Barrandon1
1Laboratoire de Dynamique des Cellule Souches, Institute of Bioengeneering, Ecole Polytechnique Fédérale de Lausanne; 2Lausanne Genomics Technologies Facility (GTF), Center for Integrative Genomics, Université de Lausanne; 3Fluidigm Corporation, South San Francisco, CA USA
P074 - Gene Expression Profiling of Duodenal Biopsies Discriminates Celiac Disease Mucosa from Normal Mucosa
Hanna Bragde1, Ulf Jansson2, Ingvar Jarlsfelt1, Jan Söderman1 1Division of Medical Diagnostics, Ryhov County Hospital, Sweden; 2Department of Pediatrics, Ryhov County Hospital, Sweden
P075 - Allele specific gene expression from single cell RNA-seq
Thomas Rygus1, Catalin Barbacioru4, Fuchou Tang2,3, Ellen Nordman4, Kaiqin Lao4, M. Azim Surani2 1Life Technologies, Germany; 2Wellcome Trust/Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK; 3BIOPIC, School of Life Sciences, Peking University, Beijing, 100871, China; 42Genetic Systems, Applied Biosystems, part of Life Technologies, 850 Lincoln Centre Drive, Foster City, CA 94404, USA


P076 – P084    HRM & Methylation Studies  

P076 - The EpiQ Chromatin Analysis Kit: A New Tool for Epigenetic Research
Viresh Patel Bio-Rad Laboratories, United States of America
P077 - Chloroplast DNA heterogeneity detection with HRM analysis
Irem Uzonur, Esma Ozsoy, Zeynep Katmer, Fatih Koyuncu, Gamze Akdeniz, Ali Rıza Atasoy Fatih University, Turkey
P078 - Semi-quantitative analysis of O6-methylguanine-DNA methyltransferase (MGMT) promoter hypermethylation by high resolution melting (HRM) real-time PCR
Kok-Siong Poon1, Lily Chiu1, Evelyn Siew-Chuan Koay1,2 1Molecular Diagnosis Centre, National University Hospital, Singapore; 2Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore
P079 - Application of High Resolution Melting to SNP genotyping of Porcine Ryanodine Receptor Gene
ANNA CASTELLO1, ANNA MERCADE2, ARMAND SANCHEZ1,2
1Departament de Ciencia Animal i dels Aliments, Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain; 2Servei Veterinari de Genetica Molecular, Facultat Veterinaria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain
P080 - DNA methylation biomarkers for noninvasive detection of prostate cancer
Kristina Daniunaite1, Feliksas Jankevicius2,3, Juozas Lazutka1, Sonata Jarmalaite1 1Faculty of Nature Sci., Vilnius University, Lithuania; 2Faculty of Medicine, Vilnius University, Lithuania; 3Vilnius University Hospital Santariskiu Clinics, Vilnius, Lithuania
P081 - Does DNA methylation of the Estrogen Receptor Alpha gene contribute to transcriptional regulation in the bovine endometrium?
Rainer W. Fürst, Heinrich H.D. Meyer, Susanne E. Ulbrich Physiology Weihenstephan, Technische Universität München, Germany
P082 - Microarray gene expression and mtDNA deletion analysis in laser dissected human muscle fibers.
Matthias Elstner1, Konrad Olszewski1, Christoph Laub1, Anne Averdam1, Lena Bruns1, Andreas Bender1, Holger Prokisch2, Thomas Klopstock1 1Ludwig-Maximilians University Munich, Germany; 2Helmholtz Zentrum Munich, Germany
P083 - From traditional RAPD-PCR to next generation HRM assisted real-time RAPD PCR
Irem Uzonur, Zeynep Katmer, Esma Ozsoy, Fatih Koyuncu, Tugba Senel Fatih University, Turkey
P084 - High Resolution Melting with Unlabelled Probe approach for the analysis of Short Sequence Tandem Repeats loci to sub-type Mycobacterium avium subsp. paratuberculosis
Matteo Ricchi, Gianluca Barbieri, Norma Arrigoni IZSLER, National Centre for Paratuberculosis, Piacenza, Italy


P085 – P090    RNAi:  microRNA – siRNA Applications  

P085 - Titer dependent cytotoxicity of viral vectors measured in real time via ECIS-technology
Jakob Müller1, Christian Thirion2, Pfaffl Michael W.1 1Technische Universität München, Germany; 2Friedrich-Baur-Institute, Klinikum der LMU, Department of Neurology, Germany
P086 - Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers.
Susanna Cirera1, Ingrid Balcells2, Peter K. Busk3 1Genetics and Bioinformatics, University of Copenhagen, LIFE, Denmark; 2Unitat de Ciència Animal, University Autonoma of Barcelona, Spain; 3Section for Sustainable Biotech, Aalborg University, Denmark
P087 - Silencing of a Superoxide Dismutase in Phaedon cochleariae (Coleoptera: Chrysomelidae) by using RNAi.
René Roberto Bodemann, Wilhelm Boland, Antje Burse Max Planck Institute for Chemical Ecology, Germany
P088 - Broad based profiling ~1150 microRNA designed in version 16 of miRBase in a single experiment using the SmartChip System from WaferGen Biosystems
Tony Dodge, alan chang, Harita Veereshlingam, Sangeetha Anandakrishnan, Sherry Wei, Roy Bohenzky WaferGen Biosystems, United States of America
P089 - ChIP-qPCR and qbasePLUS jointly identify a MYCN-activated miRNA cluster in cancer
Barbara D'haene1, Pieter Mestdagh2, Daniel Muth3, Frank Westerman3, Frank Speleman2, Jo Vandesompele1,2
1Biogazelle, Zwijnaarden, Belgium; 2Center for Medical Genetics, Ghent University, Ghent, Belgium; 3Department of Tumour Genetics, German Cancer Center, Heidelberg, Germany
P090 - LNA-based miRNA qPCR facilitates the discrimination of different stages in cancer progression in a transgenic mouse model
Kim B Barken1, Ditte Andreasen1, Rolf Søkilde1, Jan Stenvang2, Niels Tolstrup1, Thomas Litman3, Boye S Nielsen1, Peter Mouritzen1 1Exiqon, Denmark; 2Copenhagen University, LIFE, Denmark; 3Litman Consulting, Denmark


P091    late submission

P091 - qPCR for the Detection and Quantification of Adeno-Associated Virus Serotype 2 (AAV2) ITR-Sequences
Aurnhammer, Christine; Haase, Maren; Muether, Nadine; Häusl, Martin; Huber, Ingrid; Nitschko, Hans; Busch, Ulrich; Sing, Andreas; Erhardt, Anja; Baiker, Armin Organization(s): Bavarian Health and Food Safety Authority (LGL), Germany